While the SPIM Registration will continue to live within Fiji for the time being, we will mostly offer support only for this new software package. This software package is the successor to the SPIM Registration package. Both projects share a common XML data model to describe multiview datasets. Interactive viewing and annotation of the data is provided by integration with Tobias Pietzsch’s BigDataViewer. The software is designed for lightsheet fluorescence microscopy (LSFM, second box), but is applicable to any form of three or higher dimensional imaging modalities like confocal timeseries or multicolor stacks. The Multiview Reconstruction software package enables users to register, fuse, deconvolve and view multiview microscopy images (first box). Webpageįor technical details about the registration method and SPIM imaging see also SPIM Registration Method. Tomancak (2014) “Efficient Bayesian-based Multiview Deconvolution”, Nature Methods, 11(6):645-648. Tomancak (2010) “Software for bead-based registration of selective plane illumination microscopy data”, Nature Methods, 7(6):418-419. If you use it successfully for your research please be so kind to cite our work: Please note that the SPIM registration plugin available through Fiji, is based on a publication. Non-Separable Convolution: Used for the MultiView Deconvolution, available on GitHub.Separable Convolution: Used for the Difference-of-Gaussian segmentation, available on GitHub.To enable GPU hardware accelerated processing, you might want to download the native CUDA code for: The source code is available on GitHub, please also report feature requests & bugs there. Simply update Fiji and the Multiview-Reconstruction pipeline will be available under Plugins › Multiview Reconstruction › Multiview Reconstruction Application. The integration of the Multiview Reconstruction and the BigDataViewer is available through the Fiji Updater. If you’d like to help, check out the how to help guide! Download The content of this page has not been vetted since shifting away from MediaWiki.
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